Objective Lenses enable deep imaging of biological samples.
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Press Release Summary:
Intended for multiphoton excitation (MPE) and light sheet applications, XLPLN10XSVMP and XLSLPLN25XGMP support for range of refractive indices (RIs) and enable deep imaging of tissues treated with clearing agents as well as live-cell imaging and light sheet microscopy techniques. Dedicated objectives, which have 8 mm working distance and 400–1,600 nm transmittance range, also offer field number of 27.5 and correction collar. Latter allows compensation of refractive index mismatches.
Original Press Release:
Unmatched Deep Imaging of Biological Samples
Olympus launches its dedicated multiphoton excitation (MPE) objectives for deep imaging. Designed to vastly accelerate data collection from live-cell and clarified samples with high-resolution imaging, the new lenses are the clear choice for MPE and light sheet applications.
Hamburg – Dedicated to multiphoton excitation microscopy, Olympus introduces its XLPLN10XSVMP and XLSLPLN25XGMP objective lenses with an 8 mm working distance and support for a large range of refractive indices (RI). The objectives enable super-deep imaging of tissues treated with the latest clearing agents as well as live-cell imaging and light sheet microscopy techniques.
Compatible with the industry leading FVMPE-RS and FV1200MPE multiphoton microscopy systems and easily adaptable to home-build light sheet microscopes, the new objectives boast a range of features providing important benefits to researchers. Both objectives offer super-long working distances of 8 mm, providing the capability and space for structural and live-cell imaging deep within large intact samples (such as whole mouse brain), especially useful in neuroscience applications. A high transmittance range of 400-1600 nm extending into the infra-red spectrum allows deeper imaging with minimal damage to the tissue, and is well-suited to combine conventional multicolour MPE imaging with label-free methods like third- and second-harmonic generation. A field number of 27.5 provides excellent scattered light collecting abilities and a correction collar allows compensation of refractive index mismatches. Furthermore, spherical aberrations are corrected across the visible to infra-red (IR) range, with additional chromatic aberration correction in the IR range.
The XPLN10XSVMP is also extremely versatile with multi-immersion capability enabling its use with water, silicone and oil as well as clearing agents such as SCALEVIEW(1), Clarity(2) and SeeDB (RI 1.33-1.52)(3). Deep macro observation is achieved with high resolution at a large field of view (FOV – 1.8 mm at 10X magnification). The XLSLPLN25XGMP also supports the latest in clearing agents such as Clarity and SeeDB for cutting-edge research techniques, aided by a high RI immersion capability of 1.41-1.52 (SeeDB requires an RI of 1.49).
These additions to Olympus’ XLPLN series of objectives extend the company’s lead in the field of multiphoton excitation microscopy. The objectives enable the interrogation of large intact samples (such as whole-brain high-resolution imaging) and structural and molecular analysis of large assembled biological systems. The large FOV provided by the objectives enhances data collection from samples treated with the latest clearing agents by several orders of magnitude and this combined with unsurpassed flexibility and correctional capabilities makes them the clear choice for researchers using MPE or light sheet microscopy techniques.
For further information please visit www.olympus-europa.com/microscopy.
References
(1) Tomer R, Li Y, Hsueh B, Deisseroth K, Nature Protocols 9 (7), 1682-1697 (2014 - published online 19 June 2014); doi:10.1038/nprot.2014.123
(2) Chung K, Deisseroth K, Nature Methods 10, 508-513 (2013); doi 10.1038/nmeth.2481
(3) Ke MT, Fujimoto S, Imai T, Nature Neuroscience 6 (8), 1154-1161 (2013); doi: 10.1038/nn.3447
Please contact:
Olympus:
Ralf Schäfer (Marketing Communications Manager Microscopy)
Olympus Europa SE & Co. KG
Hamburg, GER
Tel: +49 (0) 40 23773 5913
Fax: +49 (0) 40 23773 505 913
E-mail: microscopy@olympus-europa.com
Web: www.olympus-europa.com/microscopy
Text:
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Alto Marketing,
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Olympus media contact in the USA:
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Edge Communications Inc.
White Plains, NY 10605
1.914.684.0959
ilene@edge-comm.net
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