Wyatt Technology's Calypso GC-MALS System Provides Fast and Accurate Measurements of Biomolecular Interactions


(SANTA BARBARA, CALIFORNIA) - Wyatt Technology Corporation, the world leader in absolute macromolecular characterization instrumentation and software, today announced that its Calypso Composition-Gradient Multi-Angle Light Scattering (CG-MALS) system achieves accurate measurements of biomolecular interactions. The Calypso offers the unique capability of determining both the affinity and binding stoichiometry for macromolecules in solution with no need for immobilization or tagging, which may influence the interaction. This is demonstrated in a new application note, titled "Measuring Antibody-Antigen Interactions with the Calypso", which is available to download free-of-charge via www.wyatt.com/files/literature/Calypso-Measuring_Antibody-Antigen_Interactions.pdf

Antibody-antigen binding, hormone-receptor interactions and many other common biomolecular interactions occur at stoichiometries other than 1:1. Conventional separation techniques, such as size exclusion chromatography MALS (SEC MALS) and field flow fractionation MALS (FFF MALS) have been extensively used to study interactions, however they are associated with certain important limitations. As the molecules become separated in the course of dilution and fractionation, they no longer interact. Additionally, upon reaching the detectors, the molecules are often not in equilibrium, neither are they in a well defined kinetic state.

The new application note illustrates how Calypso complements traditional MALS separation techniques, keeping the molecules in close proximity to probe their interactions. The analyzer was used in conjunction with an online UV/Vis concentration detector and Wyatt's DAWN HELEOS MALS instrument to provide rapid and precise measurements of the interaction between an anti-thrombin antibody (Ab) and human thrombin á (Thr). The equilibrium dissociation constant determined by the system agreed well with the manufacturer's data as measured by ELISA. In addition, both thrombin and the antibody exhibited no propensity for self-association, a finding that could not be evaluated by conventional ELISA.

Calypso employs a series of unfractionated samples of different composition or concentration in order to characterize macromolecular interactions, including reversible self- and hetero-association of proteins, reaction rates and affinities of irreversible aggregation, and virial coefficients. No special modifications, such as sample tagging or immobilization procedures, are required. Instead, samples are unlabeled and entirely in solution. Furthermore, Calypso's automation capabilities enhance productivity by improving repeatability and reliability, while minimizing time and effort.

For more information on Wyatt Technology's instruments or to obtain a copy of the new application note, please visit www.wyatt.com or email info@wyatt.com

About Wyatt

Based in Santa Barbara, California, Wyatt Technology is the world's leading provider of instruments for absolute macromolecular characterization. With over 40 years' experience developing multi-angle light scattering detectors, working with customers in the biotechnology, chemical, petrochemical, pharmaceutical, academic and government arenas, Wyatt prides itself on its entrepreneurial spirit, and the uniqueness of its offerings. The Company's groundbreaking technology and uncompromising levels of customer care make Wyatt the global hallmark in its field. For more information, please visit www.wyatt.com

For further press information please contact: Laura Browne, The Scott Partnership, 1, Whiteside, Station Road, Holmes Chapel, Cheshire, CW4 8AA, United Kingdom; Tel: + 44 1477 539539; Fax: +44 1477 539540; email to:wyatt@scottpr.com

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