Thermo Fisher Scientific adds FAIMS Capability to Ion Trap Mass Spectrometers: Precise and Reliable Results for Proteomics and Metabolism Analyses


Chromatography and Mass Spectrometry

June 06 2007 -- Thermo Fisher Scientific, the world leader in serving science, announces that the Thermo Scientific FAIMS (high-Field Asymmetric waveform Ion Mobility Spectrometry) interface is now available for the Thermo Scientific industry-leading family of ion trap mass spectrometers. The FAIMS capability eliminates interferences and significantly increases the number of the total spectra that match sequences and eliminates the need for additional time-consuming separation steps. Well-suited for proteomics applications, as much as 90% of the total FAIMS spectra have been shown to match peptide sequences. The combination of FAIMS and ion trap mass spectrometers addresses the needs of scientists working in laboratories who require more definitive results and multiple modes of separation from interferences. The new, extended capability will be displayed at the downtown Marriott Ballroom 5, daily from 7:00 - 10:00 PM during the 55th Annual ASMS Conference on Mass Spectrometry, June 3-7 in Indianapolis, IN.

Thermo Scientific ion trap mass spectrometers, when coupled with the FAIMS interface, allow users to selectively isolate target compounds based on a number of physical properties, including charge state and molecular conformation. This selection of ions occurs before the introduction of the sample into the mass spectrometer, reducing background noise, increasing selectivity and improving spectral quality.

According to Dr. Michael J. MacCoss, Assistant Professor in the Department of Genome Sciences at the University of Washington, using FAIMS on the Thermo Scientific LTQ(TM) provides the dynamic range and peak capacity of multidimensional protein identification technology (MudPIT) in a one dimensional liquid chromatographic separation. He states, "The separation of coeluting, isobaric components minimizes the number of MS/MS spectra acquired using data-dependent acquisition that are composed of multiple molecular species. FAIMS on the LTQ has substantially changed the way we think about proteomics profiling experiments."

Traditionally, up to twelve 60-minute MudPIT steps are taken in order to fully separate protein/peptide mixtures requiring a significant amount of acquisition and processing time. The FAIMS capability eliminates the need for these steps by doing direct infusion and enables the scanning of the entire mobility range in one minute with no need to do any additional separation, thus saving valuable time.

Adding the multiple degrees of separation capability of FAIMS for protein and peptide analysis enhances both selectivity and sensitivity of low-abundant proteins for identification and offers important benefits for applications such as biomarker research, protein profiling and protein structure characterization.

Separation with FAIMS prior to MS analysis reduces the number of ions introduced into the ion trap, eliminating solvent ions which complicate identification, and singly-charged ions which render little peptide information. This also significantly improves dynamic range and, when coupled with the inherent speed of the LTQ XL(TM), results in an analysis on a chromatographic timescale.

Additionally, FAIMS allows for the separation of isobaric metabolites for easy identification and confirmation of metabolites, as well as affording improved overall spectral quality by removing endogenous background ions from formulations and dosing excipients.

The FAIMS interface also works with the Thermo Scientific TSQ Quantum series triple quadrupole mass spectrometers to increase selectivity and robustness in challenging assays.

For more information about the new, combined FAIMS-Ion Trap MS platform please call +1 800-532-4752, e-mail analyze@thermofisher.com or visit www.thermo.com/ms.

Thermo Scientific is part of Thermo Fisher Scientific, the world leader in serving science.

About Thermo Fisher Scientific

Thermo Fisher Scientific Inc. (NYSE: TMO) is the world leader in serving science, enabling our customers to make the world healthier, cleaner and safer. With an annual revenue rate of more than $9 billion, we employ 30,000 people and serve over 350,000 customers within pharmaceutical and biotech companies, hospitals and clinical diagnostic labs, universities, research institutions and government agencies, as well as environmental and industrial process control settings. Serving customers through two premier brands, Thermo Scientific and Fisher Scientific, we help solve analytical challenges from routine testing to complex research and discovery. Thermo Scientific offers customers a complete range of high-end analytical instruments as well as laboratory equipment, software, services, consumables and reagents to enable integrated laboratory workflow solutions. Fisher Scientific provides a complete portfolio of laboratory equipment, chemicals, supplies and services used in healthcare, scientific research, safety and education. Together, we offer the most convenient purchasing options to customers and continuously advance our technologies to accelerate the pace of scientific discovery, enhance value for customers and fuel growth for shareholders and employees alike. Visit www.thermofisher.com.

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