Sigma-Aldrich Expands Lentiviral shRNA Gene Family Sets
ST. LOUIS, July 25 // -- Sigma-Aldrich (NASDAQ:SIAL), a leading $1.7 billion Life Science company and a member of The RNAi Consortium (TRC), is pleased to announce the expansion of shRNA gene family sets in lentiviral format for direct use in gene silencing. The MISSION(TM) TRC shRNA Human Kinases Gene Family is the second in a series of shRNA target sets that allows for rapid RNAi cell-based screens in mammalian systems not easily manipulated by traditional siRNA. The lentiviral transduction particle format eliminates the need to propagate bacterial cells, purify plasmid DNA or undertake the lengthy process of preparing virus, saving time and valuable resources. Lentiviral-based shRNA can be used to create a stable knockdown in almost any mammalian cell type, including primary and non-dividing cells.
"Studying the essential role kinases play in immune response, metabolism and cell differentiation is key to the discovery and development of new therapeutics. This collection will enhance drug discovery screens and provide insight into important intercellular communication mediated by protein kinases," said Christina Bailey, Global Strategic Market Manager, Functional Genomics. "The kinases assembled in this set are ideal for high throughput screens and the establishment of stable knockdown cell lines."
The MISSION TRC shRNA Human Kinases are also available as bacterial glycerol stocks. Additional gene family sets are available in both glycerol and lentiviral particle format.
Sigma-Aldrich is committed to advancing functional genomics through the expansion of gene family sets targeting major research areas. These leading scientific products are backed by a broad portfolio of exclusive intellectual property and have been developed utilizing the Company's state-of-the-art high throughput lentiviral production center.
Learn more about the MISSION(TM) TRC shRNA products at sigma-aldrich.com/rnai .
About Sigma-Aldrich: Sigma-Aldrich is a leading Life Science and High Technology company. Its biochemical and organic chemical products and kits are used in scientific and genomic research, biotechnology, pharmaceutical development, the diagnosis of disease and as key components in pharmaceutical and other high technology manufacturing. The Company has customers in life science companies, university and government institutions, hospitals, and in industry. Over one million scientists and technologists use its products. Sigma-Aldrich operates in 35 countries and has 7,200 employees providing excellent service worldwide. Sigma-Aldrich is committed to Accelerating Customer Success through Leadership in Life Science, High Technology and Service. For more information about Sigma-Aldrich, please visit its award- winning Web site at sigma-aldrich.com .
About TRC: TRC is comprised of principal investigators from world-class academic research institutions (Massachusetts Institute of Technology, Harvard Medical School, the Broad Institute, Whitehead Institute for Biomedical Research, Dana-Farber Cancer Institute, Massachusetts General Hospital, Washington University, Columbia University, and Academia Sinica) as well as corporate sponsoring institutions (Sigma-Aldrich, Novartis, Eli Lilly, and Bristol-Myers Squibb). As a scientific collaborator and distribution partner through agreement with MIT, Sigma-Aldrich is working with TRC to provide the scientific community with RNAi tools for functional genomics research specifically for gene function discovery and the study of disease. The MISSION TRC shRNA clone libraries will comprise a comprehensive collection of 150,000 pre-cloned lentiviral-based shRNA vector constructs targeting 15,000 human genes (MISSION TRC-Hs1.0) and 15,000 mouse genes (MISSION TRC-Mm1.0). Design and development of the TRC libraries is being led by the Broad Institute of the Massachusetts Institute of Technology (MIT) and Harvard. For more information about MISSION shRNA clone collections, please visit us online at sigma-aldrich.com/rnai .
Source: Sigma-Aldrich
CONTACT:
Sean Battles
Sigma-Aldrich
Tel: 314-286-7616
Email: sean.battles@sial.com
Web site: www.sigma-aldrich.com/
http://sigma-aldrich.com/rnai
