PCR Buffer eliminates post-amplification steps.

Press Release Summary:



Formulated with red tracker dye and precipitant in reaction buffer, ReddyMix(TM) PCR Buffer enables samples to be loaded directly onto agarose gel after thermal cycling, reducing number of pipetting and reagent handling steps in PCR testing protocols. This reduces contamination risks and enhances testing reproducibility. Utilization of mixes containing buffer allows laboratories to avoid using separate loading dyes. Product has no adverse effect on outcome of PCR.



Original Press Release:



Thermo Fisher Scientific Offers Unique Time-Saving PCR Buffer to Eliminate Post-Amplification Steps



Nucleic Acid Amplification & Biostorage

EPSOM UK, 11 May 2007 - Thermo Fisher Scientific, the world leader in serving science, has developed a unique PCR buffer that enables samples to be loaded directly onto an agarose gel after thermal cycling, thus reducing the number of pipetting and reagent handling steps in PCR testing protocols.

The Thermo Scientific ReddyMix(TM) PCR Buffer is specially formulated with a red tracker dye and precipitant in the reaction buffer. Utilisation of mixes containing this buffer allows laboratories to avoid having to buy and utilise separate loading dyes when loading PCR products on to agarose gels for electrophoresis and subsequent visualisation, thus saving both time and money.

The red dye in ReddyMix migrates between bromophenol blue and xylene cyanol at approximately 300bp, depending on the agarose concentration. Since the additives are contained in the reaction buffer rather than in the enzyme preparation, the red dye and precipitant are present at optimal concentrations in the final mix. This contrasts favourably with products that contain dye and precipitant in the enzyme formulation, as these tend to sink slowly and erratically into test plate wells, producing dye fronts that are hard to see.

By reducing pipetting and reagent handling steps in the PCR process, use of ReddyMix reduces contamination risks and enhances testing reproducibility. This means that standard tests can be set up for purposes such as screening or teaching, enabling users to be confident that results will be consistent every time.

ReddyMix has no adverse effect on the outcome of PCR, and yields are exactly the same as would be achieved with standard polymerase products. Use of the product does not interfere with downstream applications.

ReddyMix is available across the whole of Thermo Scientific's PCR reagent range.

For information on pack sizes and samples please speak to your local Thermo Scientific representative or contact
Melanie Yanek,
Reagents Product Manager
+44 (0) 1372 840536,
email: melanie.yanek@thermofisher.com
or visit http://www.thermo.com/qpcr.

Thermo Scientific is part of Thermo Fisher Scientific Inc. (NYSE: TMO), the world leader in serving science.

About Thermo Fisher Scientific:

Thermo Fisher Scientific Inc. is the world leader in serving science, enabling our customers to make the world healthier, cleaner and safer. With an annual revenue rate of more than $9 billion, we employ 30,000 people and serve over 350,000 customers within pharmaceutical and biotech companies, hospitals and clinical diagnostic labs, universities, research institutions and government agencies, as well as environmental and industrial process control settings. Serving customers through two premier brands, Thermo Scientific and Fisher Scientific, we help solve analytical challenges from routine testing to complex research and discovery. Thermo Scientific offers customers a complete range of high-end analytical instruments as well as laboratory equipment, software, services, consumables and reagents to enable integrated laboratory workflow solutions. Fisher Scientific provides a complete portfolio of laboratory equipment, chemicals, supplies and services used in healthcare, scientific research, safety and education. Together, we offer the most convenient purchasing options to customers and continuously advance our technologies to accelerate the pace of scientific discovery, enhance value for customers and fuel growth for shareholders and employees alike. Visit www.thermofisher.com

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