New SDM and Directed Evolution for Designing and Manufacturing Enzyme Molecules
Press Release Summary:
- Requires acknowledgment of the primary structure and gene encoding sequence of the enzyme
- In vitro compartmentalization is a novel strategy for protein transformation that has emerged in recent advancements
- Mimics in vivo evolution inside a cell by enclosing proteins and encoding DNAs within the compartment
Original Press Release:
Creative Enzymes Introduces Two Enzyme Engineering Methods to Accelerate Related Research
Creative Enzymes, a professional enzyme provider located in New York, USA, is always hammering away at research and trials in order to provide customers with enzyme services and products with its greatest effort. Just a while ago, the company announced the launch of its reliable enzyme engineering services through site-directed mutagenesis (SDM) and directed evolution, aiming to advance enzymology to a further extent.
In recent years, the development of structural biology and gene manipulation technology has enabled scientists to effectively modify and even begin artificially designing and manufacturing enzyme molecules of interest, thus driving the development of molecular enzyme engineering. In a nutshell, molecular enzyme engineering involves using genetic and protein engineering strategies/technologies to study the cloning, expression, and sequence analysis of genes encoding proteins. The relationship between the structure and function of enzymes is the key to further direct protein modifications and creating novel enzymes of better performance.
The current research hotspots in enzymology can be summarized as 1. the use of genetic engineering technology for the massive preparation of enzyme products and 2. the modification of natural enzymes through site-directed mutagenesis and directed evolution by in vitro compartmentalization.
Site-directed mutagenesis required acknowledgment of the primary structure and gene encoding sequence of the enzyme, thus designing mutation sites based on the spatial structure. Directed evolution by in vitro compartmentalization is a novel strategy for protein transformation that has emerged in recent advancements, which is applied when the spatial structure of the protein is unknown or when the site-directed mutation strategy is limited. It mimics in vivo evolution inside a cell by enclosing proteins and encoding DNAs within the compartment, thereby creating spatial confinement through laboratory means. The process simulates various gene mutation sites, and desired properties or functions are directly selected so that the natural evolution process of millions of years can be realized efficiently.
Directed evolution services and a variety of enzyme engineering tools can meet the client’s needs in improving enzyme activity and properties. Creative Enzymes’ directed evolution service has won the trust of thousands of customers based on its unique advantages and excellent quality. And with the technology to alter any given base in cloned DNA virtually, the company has established a reliable enzyme engineering service through site-directed mutagenesis (SDM).
“Creative Enzymes provides site-directed mutagenesis and directed evolution services with high efficiency. We offer customer-oriented directed evolution and carefully tune tools to eliminate possible shortfalls and maximize result efficiency at every stage of enzyme engineering. With our support, your research is on the accelerated track to success,” said Creative Enzymes’ senior scientist.
About Creative Enzymes
Creative Enzymes is a remarkable supplier and manufacturer of probiotics. Equipped with advanced technology platforms, Creative Enzymes is able to offer high-quality and professional probiotic products for customers. Last but not least, its products and services have been widely used to leverage research for academic institutions and pharmaceutical industries.
Contact:
Address: Shirley, NY 11967, USA
Email: contact@creative-enzymes.com
