New Mouse Lymphoma Assay (MLA) Data Processing Workbook
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Cambridge, UK. 27 - Working with Mouse Lymphoma Assay (MLA) experts, Perceptive Instruments Ltd have developed an Excel spreadsheet, designed to work with the Sorcerer Colony Counter, specifically for soft agar MLA investigations. Sorcerer is the only MLA specific colony counting system on the market.
Globally, MLA is the most widely used in vitro mammalian cell gene mutation assay. Mammalian cell cultures can be used to detect genetic mutations induced by chemical substances. Typically, MLA detects mutations at the thymidine kinase locus caused by base pair changes, frame-shifts and deletions. The assay is used routinely by contract research laboratories and research institutes.
The MLA workbook can be used to record daily culture maintenance data. Then, by counting both mutant plates and viability plates, the spreadsheet will automatically calculate the mutant frequency, mutant index and relative plating efficiency. Before you begin counting colonies, the spreadsheet can be configured for your experimental set-up. The number of positive/negative controls and test cultures are easily chosen and the spreadsheet is instantly created with the correct number of rows and columns.
The MLA workbook seamlessly integrates with the Sorcerer Colony Counter and allows accurate colony counting with instant size distribution information. The Petri-viewer optimally illuminates the soft agar MLA plates then colony count and size data is generated in approximately 0.1 seconds. Sorcerer is fast and accurate and used by many of the leading contract research laboratories across the globe.
This new soft agar MLA data processing spreadsheet is included with every Sorcerer purchase. Existing Perceptive Instruments Ltd customers with maintenance contracts or a valid warranty on their Sorcerer system should contact us for a free copy.
Perceptive Instruments Ltd, founded in 1990, develops, manufactures and supplies image analysis and data processing solutions for many areas of scientific research and industry. Our products are used primarily in the pharmaceutical & health care industries, as well as environmental monitoring, food and manufacturing industries. www.perceptive.co.uk
