New Horseradish Peroxidase Structured by Polypeptide Chains (33,890 Da)

Press Release Summary:

  • Available with an isoelectric point of pH 3–9
  • Consists of isoenzymes with a molecular weight of 44,000 Da
  • Ideal for western blotting, ELISA, and immunohistochemistry

Original Press Release:

Creative Enzymes Expands its Service Offering with The Launch of Native Horseradish Peroxidase

Creative Enzymes, a professional enzyme provider located in New York, USA, is always hammering away at research and trials in order to provide customers with as many enzyme services and products as possible. Creative Enzymes recently announced the launch of Horseradish Peroxidase for research and industrial production.

Horseradish peroxidase, abbreviated as HRP, is a single-chain polypeptide containing four disulfide bridges. It is a glycoprotein consisting of 18% carbohydrates. The composition of these carbohydrates includes galactose, arabinose, xylose, fucose, mannose, mannosamine, and galactosamine, depending on the specificity of the isoenzyme. HRP consists of several isoenzymes with a molecular weight of 44,000 Da, structured by polypeptide chains (33,890 Da), heme plus Ca2+ (700 Da), and carbohydrates (~ 9,400 Da). HRP has an isoelectric point of pH 3–9, and the optimum pH for enzyme catalysis varies slightly depending on the hydrogen donor but is mostly around pH 5.

HRP is currently the most widely used labeling enzyme for antibodies and proteins, which can be used in both localization detection and quantitative determination. It has been adopted for various biochemical applications, such as western blotting, ELISA, and immunohistochemistry, and it is applied to amplify weak signaling and enhance the detection of target molecules, such as proteins. HRP is commonly used to determine the amount of glucose and peroxide in solutions and has also been used for rapid susceptibility testing in Aspergillus fumigatus.

“Horseradish peroxidase has a wide range of roles in clinical tests. It can be used not only as an indicator in biochemical reactions but also as a labeling reagent in immune reactions (especially ELISA) and is a key raw material for enzyme-labeled antibodies. Horseradish peroxidase, a key component of the chromogenic system for multiple kits, has an important impact on the quality of these kits. Horseradish Peroxidase (HRP) at Creative Enzymes is purified for activity assays and conjugated to antibodies for ELISA, Western blot, and immunohistochemical applications,” said the marketing manager of Creative Enzymes.

Working on a project involving horseradish peroxidase? Want more detailed information? Please get in touch with Creative Enzymes at 1-516-855-7709 or email contact@creative-enzymes.com. Scientists here would be more than happy to serve you.

About Creative Enzymes

Creative Enzymes is a remarkable supplier and manufacturer in the enzymology field. Equipped with advanced technology platforms, Creative Enzymes is able to offer high-quality and professional services to customers. Its products and services have been widely used institutionally for academics and industrially for pharmaceuticals.

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