Fractionation System analyzes complex protien mixtures.
Press Release Summary:
ProteomeLab(TM) PF 2D automated system resolves thousands of proteins present in cell lysates, producing results in hours. In first dimension, chromatofocusing separates proteins according to their pI, providing information that can be related to traditional isoelectric focusing techniques. Fractions from first dimension are collected and injected into second dimension, which separates on basis of hydrophobicity. Software displays both 1D and 2D maps of expressed protein profiles.
Original Press Release:
New Automated Two-Dimensional Protein Fractionation System Enhances Proteome Analysis
Fullerton, California, December, 2002 – Beckman Coulter, Inc. introduces the ProteomeLab™ PF 2D, an automated, two-dimensional fractionation system for high-resolution analysis of complex protein mixtures. Part of the company’s new ProteomeLab family of products, the PF 2D produces results in just hours. The system effectively resolves the thousands of proteins present in cell lysates, addressing a key challenge in today’s proteomics research. In the first dimension, chromatofocusing separates the proteins according to their pI, providing information that can be related to traditional isoelectric focusing techniques. Fractions from the first dimension are collected and automatically injected into a second dimension, which separates on the basis of hydrophobicity. ProteomeLab PF 2D software displays both one- and two-dimensional maps of the expressed protein profiles in easy-to-read formats. This “in solution” approach delivers liquid fractions for further study. The PF 2D system addresses many of the problems associated with traditional proteomics research, such as detection of low abundance proteins, run-to-run reproducibility, quantitation, detection of membrane or hydrophobic proteins, detection of basic proteins and detection of very low and very high molecular weight proteins. The automated system also requires less time and manpower than traditional, labor-intensive techniques. “Identification and characterization of total proteomes presents a huge challenge,” explains John Hobbs, Strategic Market Manger for Beckman Coulter. “A typical cell lysate can contain as many as 10,000 different proteins over a wide range of concentration. Traditional techniques such as 2-D gel electrophoresis are widely used and can provide high resolution, but they also have significant shortcomings when it comes to meeting this challenge.” Beckman Coulter, Inc. is a global biomedical company, headquartered in Fullerton, California. The company develops and markets instruments, chemistries, software and supplies that simplify and automate laboratory processes throughout the biomedical testing continuum. Through pioneering medical research and drug discovery, specialty testing and patient care diagnostics, Beckman Coulter supports all phases of the battle against disease. Annual sales for the company totaled $2 billion in 2001, with 62% of this amount generated by recurring revenue from supplies, test kits and services. For more information, access the Beckman Coulter World Wide Web Information Service at www.beckmancoulter.com or call (800) 742-2345 (U.S.). Fax (800) 643-4366 (U.S.). From outside the U.S. call (714) 871-4848, or Fax (714) 773-6611. Please send READER INQUIRIES from this news release directly to Beckman Coulter, c/o K. Delgado, Harte Hanks, 1753 South Pointe Avenue, Ontario, CA 91761. Or e-mail to k_delgado@harte-hanks.com.
