IDT Introduces Downloadable qPCR User Guide
Providing a MIQE compliant overview of this essential research technique
CORALVILLE, IA - December 8 2010. Integrated DNA Technologies (IDT), the world leader in oligonucleotide synthesis, has developed an extensive quantitative real-time polymerase chain reaction (qPCR) user guide, which is available to download for free from idtdna.com. Providing user guidance on the entire qPCR process - from RNA isolation to data analysis - the manual covers the basics of experimental set-up, performance and analysis. Specific information on 5' nuclease assays, including re-suspensions and qPCR protocols are also supplied, as well as a troubleshooting section which discusses commonly encountered issues. The entire document is written in compliance with MIQE guidelines: minimum information for publication of quantitative real-time PCR1.
IDT offers a broad range of PrimeTime qPCR assay products including customized primers and probes, in addition to the free online design suite - SciTools. For further information or to download the qPCR application guide, please visit idtdna.com.
1. Bustin, S.A. et al. The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. Clin Chem, 2009. 55(4): p. 611-22.
About IDT
Integrated DNA Technologies (IDT) is the largest supplier of custom nucleic acids in the United States, serving academic, government, and commercial researchers in biotechnology, clinical diagnostics, and pharmaceutical development. IDT's primary business is the manufacture of custom, synthetic DNA and RNA oligonucleotides. Today, IDT synthesizes and ships an average of 36,000 custom oligos per day to more than 86,000 customers worldwide. IDT manufacturing locations include facilities in Coralville, Iowa; San Diego, Calif.; and Leuven, Belgium. For more information, visit idtdna.com.
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