No-Wash Calcium Flux Assay is designed for GPCR screening.
August 15, 2006 -
Using homogenous mix-and-read, no-wash protocols, FLIPR Calcium 4 Assay Kit provides screening technique for GPCRs in pharmaceutical drug discovery. It includes masking technology and detects intracellular calcium flux from different cell types and receptors. Kits offer different formulations to address broad range of targets, including chemokines, small peptides, and transient, endogenous, and low-expression receptors.
Original Press release
Molecular Devices Corp.
1311 Orleans Dr.
Sunnyvale, CA, 94089-1136
Molecular Devices Introduces Its Next-Generation No-Wash, Cell-Based Calcium Assay Kit for GPCR Screening
SUNNYVALE, Calif., July 10 -- Molecular Devices Corporation (NASDAQ:MDCC), a leader in innovative solutions for drug discovery and life sciences research, today introduced its next-generation no-wash calcium flux assay -- the FLIPR Calcium 4 Assay Kit. Molecular Devices' cell based, no-wash calcium kits, first introduced in 1999, have become the standard for GPCR screening in laboratories worldwide. This new kit includes a masking technology licensed exclusively by Molecular Devices from Bayer AG (Leverkusen, Germany; Patent Nos. US 6,420,183, EP 0906572).
Calcium flux assays are considered one of the most important screening techniques for GPCRs in pharmaceutical drug discovery, and all major pharmaceutical companies utilize Molecular Devices' FLIPR system for these assays. The FLIPR Calcium Assay Kits -- Calcium, Calcium 3 and now Calcium 4 -- use homogenous mix-and-read, no-wash protocols. These kits accurately and easily detect intracellular calcium flux from different cell types and receptors. The kits offer different formulations to address a broad range of targets, including challenging systems, such as chemokines, for small peptides, and transient, endogenous and low-expression receptors that are difficult to assay with standard methods.
"Our vision is to continually innovate our masking dye technology to ensure that researchers have a range of assay configurations to use with the complex family of GPCRs. The FLIPR Calcium 4 Assay Kit is the latest addition to our calcium assay options," stated Susan Clark, Director of Reagents Marketing at Molecular Devices. "This kit contains a novel masking dye, which combines higher light extinction in the extracellular solution, contributing to an overall lower background and higher signal that benefits assays with especially small calcium responses." Clark continued, "Furthermore, the inert nature of this novel masking dye results in reduction of interference between ligands or targets and the dye."
The FLIPR fluorometric imaging plate reader and the FlexStation(R) benchtop microplate reader are optimal instruments to run the FLIPR calcium assays for rapid detection and analysis. The throughput is increased by eliminating labor-intensive wash steps and is conducive to automation for high-throughput screening as it functions at room temperature.
Molecular Devices Corporation is a leading supplier of high-performance bioanalytical measurement systems that accelerate and improve drug discovery and other life sciences research. The Company's systems and consumables enable pharmaceutical and biotechnology companies to leverage advances in genomics, proteomics and parallel chemistry to facilitate the high-throughput and cost-effective identification and evaluation of drug candidates. The Company's solutions are based on its advanced core technologies that integrate its expertise in engineering, molecular and cell biology and chemistry. Molecular Devices enables its customers to improve research productivity and effectiveness, which ultimately accelerates the complex process of discovering and developing new drugs.
CONTACT: Tim Harkness of Molecular Devices Corporation, +1-408-747-3533