ThomasNet News Logo
Sign Up | Log In | ThomasNet Home | Promote Your Business

Universal Blocking Oligos optimize target capture experiments.

Print | 
Email |  Comment   Share  
December 3, 2013 - By incorporating xGenŽ Universal Blocking Oligos into target capture experiments, users can minimize complexity and maximize throughput without degradation of performance. Oligos are able to bind to all indexed adapters using single oligonucleotide sequence, removing need for multiple oligos. This enables researchers to readily multiplex experiments for enrichment of thousands of samples.

Integrated DNA Technologies Launches xGen® Universal Blocking Oligos


Integrated DNA Technologies, Inc
1710 Commercial Park
Coralville, IA, 52241
USA



Press release date: November 21, 2013

Enhancing the performance of target capture experiments

CORALVILLE, IA – Integrated DNA Technologies (IDT), the world leader in oligonucleotide synthesis, has launched the latest addition to its portfolio of next generation sequencing (NGS) products, xGenŽ Universal Blocking Oligos. By incorporating these oligos into target capture experiments, users can decrease the complexity and increase throughput of their target capture experiment without degradation of performance.

Blocking oligos are commonly used to enhance the performance of capture experiments, through the prevention of cross-hybridization between adapter sequences. Adapters ligated to the DNA library before enrichment can hybridize to each other and cause an increase in off-target reads. Blocking oligos bind to these adapters to prevent non-specific binding. However, when multiple samples are processed simultaneously, each adapter requires a unique blocking oligo, increasing the complexity of the experiment. xGen Universal Blocking Oligos are able to bind to all indexed adapters using a single oligonucleotide sequence, removing the need for multiple oligos. As a result, researchers can more readily multiplex their experiments for cost-effective enrichment of 1000s of samples.

Universal Blocking Oligos join xGen LockdownŽ Probes, which are individually synthesized 60ƒ{120 nucleotide 5’-biotinylated capture oligos, as part of a growing offering of NGS products. Each probe is designed to enable high depth sequencing and analysis of specific regions of the genome. Whether used with custom capture panels, or for supplementing existing panels, xGen Lockdown Probes provide approximately 5000-fold enrichment, with high coverage over targeted regions and minimal GC bias.

xGen Target Capture products are ideal for users who want to focus on relevant genomic regions or transcripts of interest, or to detect single nucleotide polymorphisms, insertions/deletions, and other genetic variants using NGS.

For more information please visit www.idtdna.com. Follow us on twitter @idtdna for real-time updates and insights.

About IDT
Integrated DNA Technologies (IDT) is the largest supplier of custom nucleic acids in the world, serving academic, government, and commercial researchers in biotechnology, clinical diagnostics, and pharmaceutical development. IDT's primary business is the manufacture of custom, synthetic DNA and RNA oligonucleotides. Today, IDT synthesizes and ships an average of 36,000 custom oligos per day to more than 86,000 customers worldwide. IDT manufacturing locations include facilities in Coralville, Iowa; San Diego, Calif.; Leuven, Belgium; and Singapore. For more information visit www.idtdna.com.

Integrated DNA Technologies
800-328-2661 (US & Canada)
+1 319-626-8400 (outside US)
productinfo@idtdna.com
www.idtdna.com





Print | 
Email |  Comment   Share  
Contacts: View detailed contact information.


 

Post a comment about this story

Name:
E-mail:
(your e-mail address will not be posted)
Comment title:
Comment:
To submit comment, enter the security code shown below and press 'Post Comment'.
 



 See related product stories
More .....
 See more product news in:
Laboratory and Research Supplies and Equipment
Materials and Material Processing
 More New Product News from this company:
Assay Plates streamline qPCR analysis.
More ....
| Featured Manufacturing Jobs
 Other News from this company:
IDT and SGI-DNA Broaden Their Collaboration to Provide Synthetic DNA Products up to 2 Mbp
IDT Increases qPCR Sensitivity and Specificity with Its Double-Quenched(TM) Probes
IDT to Exhibit and Present at qPCR 2011
IDT Introduces Downloadable qPCR User Guide
IDT Enters Exclusive Agreement with 454 Life Sciences to Provide Custom Primers for GS FLX Titanium Chemistry
More ....
 Tools for you
Watch Company 
View Company Profile
Company web site
More news from this company
E-mail this story to a friend
Save Story
Search for suppliers of
Scientific Equipment
Oligomers
DNA Sequencing Reagents
Join the forum discussion at:
Hard to Handle
Tools of the Trade


Home  |  My ThomasNet News®  |  Industry Market Trends®  |  Submit Release  |  Advertise  |  Contact News  |  About Us
Brought to you by Thomasnet.com        Browse ThomasNet Directory

Copyright © 2014 Thomas Publishing Company. All Rights Reserved.
Terms of Use - Privacy Policy



Error close

Please enter a valid email address