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ASTM Pesticides Committee approves latest biofilm standard.

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February 24, 2009 - Developed by Committee E35 on Pesticides and Alternative Control Agents, Standard E2647 describes how to grow repeatable P. aeruginosa biofilm using drip flow biofilm reactor. Goal is to grow biofilm that is relevant to environment where disinfectant will be applied, then test disinfectant under real use conditions. Biofilm grown in drip flow reactor could represent biofilms found in cooling towers, produce sprayers, food processing conveyor belts, and in lungs infected with cystic fibrosis.

ASTM International Pesticides Committees Approves Latest Biofilm Standard

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ASTM International
100 Barr Harbor Dr., Box C700
West Conshohocken, PA, 19428-2959

Press release date: February 19, 2009

W. CONSHOHOCKEN, Pa., 19 February 2009-Biofilm, which is defined as microorganisms living in a self-organized cooperative community, is still under the radar for many industry, medical, professional and regulatory agencies. A newly approved ASTM International standard is the latest in a series of biofilm standards being developed by Committee E35 on Pesticides and Alternative Control Agents.

The new standard, E2647, Test Method for Quantification of a Pseudomonas aeruginosa Biofilm Grown Using a Drip Flow Biofilm Reactor with Low Shear and Continuous Flow, is under the jurisdiction of Subcommittee E35.15 on Antimicrobial Agents.

The standard describes how to grow a repeatable P. aeruginosa biofilm using a drip flow biofilm reactor. "The goal is to grow a biofilm that is relevant to the environment where a disinfectant will be applied, then test the disinfectant under real use conditions," says Darla Goeres, assistant research professor, Center for Biofilm Engineering, Montana State University, and an E35 member. Biofilm grown in a drip flow reactor could represent biofilms found in a wide variety of places, including cooling towers, produce sprayers, on food processing conveyor belts, on catheters and in lungs infected with cystic fibrosis.

"The primary user of this standard is any industrial, government and/or academic laboratory interested in conducting biofilm research," says Goeres. "In particular, the drip flow reactor has generated a lot of interest with companies and research laboratories interested in medically relevant biofilms." Goeres also notes that an important difference between bacteria in suspension and biofilm bacteria is that biofilm is tolerant to high concentrations of disinfectants or antibiotics.

Currently, E35.15 is working on a guide that will describe properties of the various biofilm growth methods and issues that must be considered when working with biofilm bacteria.

ASTM International standards are available for purchase from Customer Service (phone: 610-832-9585; or at

For technical Information, contact Darla Goeres, Center for Biofilm Engineering, Montana State University, Bozeman, Mont. (phone: 406-994-2440; Committee E35 meets April 20-23 during April committee week in Vancouver, British Columbia, Canada.

ASTM International welcomes and encourages participation in the development of its standards. ASTM's open consensus process, using advanced Internet-based standards development tools, ensures worldwide access for all interested individuals. For more information on becoming an ASTM member, please contact Jennifer Rodgers, ASTM International (phone: 610-832-9694;

Established in 1898, ASTM International is one of the largest international standards development and delivery systems in the world. ASTM International meets the World Trade Organization (WTO) principles for the development of international standards: coherence, consensus, development dimension, effectiveness, impartiality, openness, relevance and transparency. ASTM standards are accepted and used in research and development, product testing, quality systems and commercial transactions around the globe.
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